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Abstract

The present work was carried out to study effect of different culture media on the mycelial growth of antagonistic fungi of pea (Pisum sativum L.)Five different culture media were used for isolation, growth and physiological studies of antagonists. Significant effect was shown by culture media on mycelial growth, of the Trichoderma species. Potato Dextrose Agar (PDA) medium supporting maximum growth rate (45.43 mm) and minimum growth rate was observed on water ager medium (9 mm) for all the biocontrol agents. Best growth was showed by T. harzianum by utilizing all culture medium followed by Trichoderma longibrachiatum and T. viride. The maximum growth rate was observed in case of T. harzianum on PDA medium and minimum growth was in case of T. longibrachiatum and T. viride on cornmeal agar medium.

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