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Abstract
A simple, Precise, Accurate method was developed for the estimation of Rosuvastatin by RP-HPLC technique. Chromatographic conditions used are stationary phase BDS (150mm*4.6mm 5m.) Mobile phase KH2PO4: Acetonitrile in the ratio of 75:25 and flow rate was maintained at 1.0ml/min, detection wave length was 240 nm, column temperature was set to 30oC and diluent was mobile phase Conditions were finalized as optimized method. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 1.17 for repeatability and 0.85 for intermediate precision.LOD and LOQ are 0.11µg/ml and 0.34µg/ml respectively. By using above method assay of marketed formulation was carried out 99.96% was present. Degradation studies of Rosuvastatin were done, in all condition purity threshold was more than purity angle and within the acceptable range. Full length method was not performed; if it is done this method can be used for routine analysis of Rosuvastatin